AustralieFrV1, Main, Exploration, bibRecord, 003038

Clinical Performance of Aspergillus PCR for Testing Serum and Plasma: a Study by the European Aspergillus PCR Initiative

Identifieur interne : 003038 ( Main/Exploration ); précédent : 003037; suivant : 003039

Clinical Performance of Aspergillus PCR for Testing Serum and Plasma: a Study by the European Aspergillus PCR Initiative

Auteurs : P. Lewis White [Royaume-Uni] ; Rosemary A. Barnes [Royaume-Uni] ; Jan Springer [Allemagne] ; Lena Klingspor [Suède] ; Manuel Cuenca-Estrella [Espagne] ; C. Oliver Morton [Australie] ; Katrien Lagrou [Belgique] ; Stéphane Bretagne [France] ; Willem J. G. Melchers [Pays-Bas] ; Carlo Mengoli [Italie] ; J. Peter Donnelly [Pays-Bas] ; Werner J. Heinz [Allemagne] ; Juergen Loeffler [Allemagne]

Source :

Journal of Clinical Microbiology [ 0095-1137 ] ; 2015.

RBID : PMC:4540904

Descripteurs français

KwdFr :
- ADN fongique (sang), Adulte, Adulte d'âge moyen, Aspergillose (diagnostic), Aspergillose (microbiologie), Aspergillus (génétique), Aspergillus (isolement et purification), Facteurs temps, Femelle, Humains, Jeune adulte, Mâle, Plasma sanguin (microbiologie), Prélèvement biologique (), Réaction de polymérisation en chaîne (), Sensibilité et spécificité, Sujet âgé, Techniques de diagnostic moléculaire (), Études cas-témoins, Études rétrospectives.
MESH :
- diagnostic : Aspergillose.
- génétique : Aspergillus.
- isolement et purification : Aspergillus.
- microbiologie : Aspergillose, Plasma sanguin.
- sang : ADN fongique.
- Adulte, Adulte d'âge moyen, Facteurs temps, Femelle, Humains, Jeune adulte, Mâle, Prélèvement biologique, Réaction de polymérisation en chaîne, Sensibilité et spécificité, Sujet âgé, Techniques de diagnostic moléculaire, Études cas-témoins, Études rétrospectives.

English descriptors

KwdEn :
- Adult, Aged, Aspergillosis (diagnosis), Aspergillosis (microbiology), Aspergillus (genetics), Aspergillus (isolation & purification), Case-Control Studies, DNA, Fungal (blood), Female, Humans, Male, Middle Aged, Molecular Diagnostic Techniques (methods), Plasma (microbiology), Polymerase Chain Reaction (methods), Retrospective Studies, Sensitivity and Specificity, Specimen Handling (methods), Time Factors, Young Adult.
MESH :
- chemical , blood : DNA, Fungal.
- diagnosis : Aspergillosis.
- genetics : Aspergillus.
- isolation & purification : Aspergillus.
- methods : Molecular Diagnostic Techniques, Polymerase Chain Reaction, Specimen Handling.
- microbiology : Aspergillosis, Plasma.
- Adult, Aged, Case-Control Studies, Female, Humans, Male, Middle Aged, Retrospective Studies, Sensitivity and Specificity, Time Factors, Young Adult.

Abstract

Aspergillus PCR testing of serum provides technical simplicity but with potentially reduced sensitivity compared to whole-blood testing. With diseases for which screening to exclude disease represents an optimal strategy, sensitivity is paramount. The associated analytical study confirmed that DNA concentrations were greater in plasma than those in serum. The aim of the current investigation was to confirm analytical findings by comparing the performance of Aspergillus PCR testing of plasma and serum in the clinical setting. Standardized Aspergillus PCR was performed on plasma and serum samples concurrently obtained from hematology patients in a multicenter retrospective anonymous case-control study, with cases diagnosed according to European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) consensus definitions (19 proven/probable cases and 42 controls). Clinical performance and clinical utility (time to positivity) were calculated for both kinds of samples. The sensitivity and specificity for Aspergillus PCR when testing serum were 68.4% and 76.2%, respectively, and for plasma, they were 94.7% and 83.3%, respectively. Eighty-five percent of serum and plasma PCR results were concordant. On average, plasma PCR was positive 16.8 days before diagnosis and was the earliest indicator of infection in 13 cases, combined with other biomarkers in five cases. On average, serum PCR was positive 10.8 days before diagnosis and was the earliest indicator of infection in six cases, combined with other biomarkers in three cases. These results confirm the analytical finding that the sensitivity of Aspergillus PCR using plasma is superior to that using serum. PCR positivity occurs earlier when testing plasma and provides sufficient sensitivity for the screening of invasive aspergillosis while maintaining methodological simplicity.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4540904

DOI: 10.1128/JCM.00905-15
PubMed: 26085618
PubMed Central: 4540904

Affiliations:

Allemagne, Australie, Belgique, Espagne, France, Italie, Pays-Bas, Royaume-Uni, Suède
Bavière, Communauté de Madrid, District de Basse-Franconie, Gueldre, Svealand, Île-de-France
Madrid, Nimègue, Paris, Stockholm, Wurtzbourg

Le document en format XML

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<author><name sortKey="Morton, C Oliver" sort="Morton, C Oliver" uniqKey="Morton C" first="C. Oliver" last="Morton">C. Oliver Morton</name>
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<author><name sortKey="Lagrou, Katrien" sort="Lagrou, Katrien" uniqKey="Lagrou K" first="Katrien" last="Lagrou">Katrien Lagrou</name>
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<author><name sortKey="Bretagne, Stephane" sort="Bretagne, Stephane" uniqKey="Bretagne S" first="Stéphane" last="Bretagne">Stéphane Bretagne</name>
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<country xml:lang="fr">France</country>
<wicri:regionArea>Department of Microbiology and Immunology, Paris Diderot University, Saint Louis Hospital-APHP, Paris</wicri:regionArea>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
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<author><name sortKey="Melchers, Willem J G" sort="Melchers, Willem J G" uniqKey="Melchers W" first="Willem J. G." last="Melchers">Willem J. G. Melchers</name>
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<author><name sortKey="Mengoli, Carlo" sort="Mengoli, Carlo" uniqKey="Mengoli C" first="Carlo" last="Mengoli">Carlo Mengoli</name>
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<wicri:regionArea>University of Padua, Padua</wicri:regionArea>
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<author><name sortKey="Donnelly, J Peter" sort="Donnelly, J Peter" uniqKey="Donnelly J" first="J. Peter" last="Donnelly">J. Peter Donnelly</name>
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<wicri:regionArea>Radboud University Nijmegen Medical Centre, Nijmegen</wicri:regionArea>
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<author><name sortKey="Heinz, Werner J" sort="Heinz, Werner J" uniqKey="Heinz W" first="Werner J." last="Heinz">Werner J. Heinz</name>
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<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>University of Würzburg Medical Center, Würzburg</wicri:regionArea>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term>
<term>Aged</term>
<term>Aspergillosis (diagnosis)</term>
<term>Aspergillosis (microbiology)</term>
<term>Aspergillus (genetics)</term>
<term>Aspergillus (isolation & purification)</term>
<term>Case-Control Studies</term>
<term>DNA, Fungal (blood)</term>
<term>Female</term>
<term>Humans</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Molecular Diagnostic Techniques (methods)</term>
<term>Plasma (microbiology)</term>
<term>Polymerase Chain Reaction (methods)</term>
<term>Retrospective Studies</term>
<term>Sensitivity and Specificity</term>
<term>Specimen Handling (methods)</term>
<term>Time Factors</term>
<term>Young Adult</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (sang)</term>
<term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Aspergillose (diagnostic)</term>
<term>Aspergillose (microbiologie)</term>
<term>Aspergillus (génétique)</term>
<term>Aspergillus (isolement et purification)</term>
<term>Facteurs temps</term>
<term>Femelle</term>
<term>Humains</term>
<term>Jeune adulte</term>
<term>Mâle</term>
<term>Plasma sanguin (microbiologie)</term>
<term>Prélèvement biologique ()</term>
<term>Réaction de polymérisation en chaîne ()</term>
<term>Sensibilité et spécificité</term>
<term>Sujet âgé</term>
<term>Techniques de diagnostic moléculaire ()</term>
<term>Études cas-témoins</term>
<term>Études rétrospectives</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>DNA, Fungal</term>
</keywords>
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</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Aspergillose</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Aspergillus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Aspergillus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Aspergillus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Aspergillus</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term>
<term>Polymerase Chain Reaction</term>
<term>Specimen Handling</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Aspergillose</term>
<term>Plasma sanguin</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Aspergillosis</term>
<term>Plasma</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>ADN fongique</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Adult</term>
<term>Aged</term>
<term>Case-Control Studies</term>
<term>Female</term>
<term>Humans</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Retrospective Studies</term>
<term>Sensitivity and Specificity</term>
<term>Time Factors</term>
<term>Young Adult</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Facteurs temps</term>
<term>Femelle</term>
<term>Humains</term>
<term>Jeune adulte</term>
<term>Mâle</term>
<term>Prélèvement biologique</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Sensibilité et spécificité</term>
<term>Sujet âgé</term>
<term>Techniques de diagnostic moléculaire</term>
<term>Études cas-témoins</term>
<term>Études rétrospectives</term>
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<front><div type="abstract" xml:lang="en"><p><named-content content-type="genus-species">Aspergillus</named-content>
 PCR testing of serum provides technical simplicity but with potentially reduced sensitivity compared to whole-blood testing. With diseases for which screening to exclude disease represents an optimal strategy, sensitivity is paramount. The associated analytical study confirmed that DNA concentrations were greater in plasma than those in serum. The aim of the current investigation was to confirm analytical findings by comparing the performance of <named-content content-type="genus-species">Aspergillus</named-content>
 PCR testing of plasma and serum in the clinical setting. Standardized <named-content content-type="genus-species">Aspergillus</named-content>
 PCR was performed on plasma and serum samples concurrently obtained from hematology patients in a multicenter retrospective anonymous case-control study, with cases diagnosed according to European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) consensus definitions (19 proven/probable cases and 42 controls). Clinical performance and clinical utility (time to positivity) were calculated for both kinds of samples. The sensitivity and specificity for <named-content content-type="genus-species">Aspergillus</named-content>
 PCR when testing serum were 68.4% and 76.2%, respectively, and for plasma, they were 94.7% and 83.3%, respectively. Eighty-five percent of serum and plasma PCR results were concordant. On average, plasma PCR was positive 16.8 days before diagnosis and was the earliest indicator of infection in 13 cases, combined with other biomarkers in five cases. On average, serum PCR was positive 10.8 days before diagnosis and was the earliest indicator of infection in six cases, combined with other biomarkers in three cases. These results confirm the analytical finding that the sensitivity of <named-content content-type="genus-species">Aspergillus</named-content>
 PCR using plasma is superior to that using serum. PCR positivity occurs earlier when testing plasma and provides sufficient sensitivity for the screening of invasive aspergillosis while maintaining methodological simplicity.</p>
</div>
</front>
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</TEI>
<affiliations><list><country><li>Allemagne</li>
<li>Australie</li>
<li>Belgique</li>
<li>Espagne</li>
<li>France</li>
<li>Italie</li>
<li>Pays-Bas</li>
<li>Royaume-Uni</li>
<li>Suède</li>
</country>
<region><li>Bavière</li>
<li>Communauté de Madrid</li>
<li>District de Basse-Franconie</li>
<li>Gueldre</li>
<li>Svealand</li>
<li>Île-de-France</li>
</region>
<settlement><li>Madrid</li>
<li>Nimègue</li>
<li>Paris</li>
<li>Stockholm</li>
<li>Wurtzbourg</li>
</settlement>
</list>
<tree><country name="Royaume-Uni"><noRegion><name sortKey="White, P Lewis" sort="White, P Lewis" uniqKey="White P" first="P. Lewis" last="White">P. Lewis White</name>
</noRegion>
<name sortKey="Barnes, Rosemary A" sort="Barnes, Rosemary A" uniqKey="Barnes R" first="Rosemary A." last="Barnes">Rosemary A. Barnes</name>
</country>
<country name="Allemagne"><region name="Bavière"><name sortKey="Springer, Jan" sort="Springer, Jan" uniqKey="Springer J" first="Jan" last="Springer">Jan Springer</name>
</region>
<name sortKey="Heinz, Werner J" sort="Heinz, Werner J" uniqKey="Heinz W" first="Werner J." last="Heinz">Werner J. Heinz</name>
<name sortKey="Loeffler, Juergen" sort="Loeffler, Juergen" uniqKey="Loeffler J" first="Juergen" last="Loeffler">Juergen Loeffler</name>
</country>
<country name="Suède"><region name="Svealand"><name sortKey="Klingspor, Lena" sort="Klingspor, Lena" uniqKey="Klingspor L" first="Lena" last="Klingspor">Lena Klingspor</name>
</region>
</country>
<country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Cuenca Estrella, Manuel" sort="Cuenca Estrella, Manuel" uniqKey="Cuenca Estrella M" first="Manuel" last="Cuenca-Estrella">Manuel Cuenca-Estrella</name>
</region>
</country>
<country name="Australie"><noRegion><name sortKey="Morton, C Oliver" sort="Morton, C Oliver" uniqKey="Morton C" first="C. Oliver" last="Morton">C. Oliver Morton</name>
</noRegion>
</country>
<country name="Belgique"><noRegion><name sortKey="Lagrou, Katrien" sort="Lagrou, Katrien" uniqKey="Lagrou K" first="Katrien" last="Lagrou">Katrien Lagrou</name>
</noRegion>
</country>
<country name="France"><region name="Île-de-France"><name sortKey="Bretagne, Stephane" sort="Bretagne, Stephane" uniqKey="Bretagne S" first="Stéphane" last="Bretagne">Stéphane Bretagne</name>
</region>
</country>
<country name="Pays-Bas"><region name="Gueldre"><name sortKey="Melchers, Willem J G" sort="Melchers, Willem J G" uniqKey="Melchers W" first="Willem J. G." last="Melchers">Willem J. G. Melchers</name>
</region>
<name sortKey="Donnelly, J Peter" sort="Donnelly, J Peter" uniqKey="Donnelly J" first="J. Peter" last="Donnelly">J. Peter Donnelly</name>
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<country name="Italie"><noRegion><name sortKey="Mengoli, Carlo" sort="Mengoli, Carlo" uniqKey="Mengoli C" first="Carlo" last="Mengoli">Carlo Mengoli</name>
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Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003038 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003038 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Asie
   |area=    AustralieFrV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:4540904
   |texte=   Clinical Performance of Aspergillus PCR for Testing Serum and Plasma: a Study by the European Aspergillus PCR Initiative
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:26085618" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a AustralieFrV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Dec 5 10:43:12 2017. Site generation: Tue Mar 5 14:07:20 2024

Serveur d'exploration sur les relations entre la France et l'Australie

Clinical Performance of Aspergillus PCR for Testing Serum and Plasma: a Study by the European Aspergillus PCR Initiative

Clinical Performance of Aspergillus PCR for Testing Serum and Plasma: a Study by the European Aspergillus PCR Initiative

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur les relations entre la France et l'Australie
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.